Journal: Molecules

Article Title: Design and Construction of a Synthetic Nanobody Library: Testing Its Potential with a Single Selection Round Strategy

doi: 10.3390/molecules28093708

Figure Lengend Snippet: Library design. ( a ) Sequence design at the amino acid level. Positions chosen for randomization are shown as “X” in bold. The CDR sequences are highlighted in colors (blue, green and red for CDRs 1, 2 and 3, respectively), while the framework region is shown in light gray; ( b ) 3D model of a representative library nanobody based on the cAbBCII10 crystal structure (PDB: 3DWT). CDRs are colored following the same code as in panel ( a ). The colored spheres at the alpha carbons in CDRs represent the randomized positions, while gray spheres represent CDR positions that were kept fixed. ( c ) Nucleotide sequence with degenerate codons, colored by CDR; ( d ) degenerate codons used in library design and their encoded amino acids, showing also the numbers of resulting codons for each amino acid type.

Article Snippet: The Degenerate Codon Designer online tool ( https://www.novoprolabs.com/tools/degenerate-codon-designer , NovoPro, Shanghai, China, last accessed on 20 January 2023) was used for codon analyses.

Techniques: Sequencing